Zhang Lin took out the basket from the liquid nitrogen tank, then took out a cryopreservation tube from the basket, and then carefully put the basket back, because there were still many cryopreservation tubes of other cells in the basket. Zhang Lin sealed it again

After locking the lid of the liquid nitrogen tank, a series of patient and meticulous operations began.

He first placed the frozen storage tube in a water bath and quickly thawed it with 37C hot water. He then changed the centrifuge tube and centrifuged it for five minutes. Then he sat down at the ultra-clean workbench and heated the centrifuge tube nozzle with an alcohol lamp.

Bake and sterilize, discard the waste liquid, add fresh culture medium with a pipette, pipe and mix repeatedly...

Gu Jun had been watching carefully from the side. If he had any questions, he asked Senior Brother Zhang for advice and learned a lot.

For example, the speed control of the centrifuge, the suction and discharge control of the pipette, etc., any sloppiness in these recovery operations will affect the survival rate and proliferation rate of the cells.

Finally, Zhang Lin injected the cell suspension into the culture dish, added an appropriate amount of culture medium, and placed it in a 37C, 5% CO2 cell culture incubator.

"Just wait, it will take at least two days." Zhang Lin took off his mask and pushed up his glasses, "I suggest you keep it for a week, so that the tumor formation rate can be guaranteed."

"Try a batch first if you have raised them for two days." Gu Jun shrugged and said, there is no way, there is not enough time, the system shows that his condition will worsen in 30 days, if true, it may be difficult to even get out of bed and walk by then.

He added: "Senior brother, are you going out for a meal tonight? I'll treat you."

"No need." Zhang Lin waved his hand, "I have to feed the mice medicine at night. It's my treat."

…

Gu Jun spent the next two days on campus. In addition to hanging out with friends, he first read and reviewed and prepared for make-up exams. It would be quite troublesome if he had to repeat the grade if he couldn't die; second, he went there every day.

The experimental building takes care of the cells and replaces the culture medium once.

On the morning of the third day, Zhang Lin came to see it in person and said that the cells were barely growing. He helped with subculturing and preparing the cell suspension.

Gu Jun went to the school's experimental animal center. This is actually a large breeding base located a few hundred meters outside the school campus. It is very lonely, maybe because it is filled with the stench of various animals.

He bought five nude mice, each costing more than 100 yuan, which was quite expensive. Fortunately, he still had 20,000 yuan in savings, but he couldn't last long.

When money is used, you will regret it. Gu Jun really regrets donating the money so early... The person is still alive, but the money is gone.

Ahem. Each of these nude mice is only about the size of two or three fingers put together. They have no hair and are packed in a sterile box. Once they come into contact with the bacterial environment outside, they will easily become infected and get sick and die. Therefore.

Daily feeding and experimental processes need to be carried out in a barrier environment.

The barrier environment laboratories of Dongda are located in the Experimental Animal Research Center. This five-story yellow brick building stands next to the experimental building.

Gu Jun and Zhang Lin met in the lobby on the first floor of the research center and booked the right to use a small nude mouse room for one hour. This small room is only 21 square meters and is divided into a front room and a back room. The front room has a laboratory bench and instruments.

, the back room is the breeding room.

Before entering, they and all the items they brought had to undergo strict ultraviolet sterilization and disinfection. The two of them set foot in the nude mouse room wearing a special full-body protective suit, masks, gloves, foot gloves and other equipment.

.

Soon, at the experimental table in the front room, Gu Jun marked five nude mice with the punching method, and then injected tumor cells subcutaneously into the right side of their buttocks one by one.

Zhang Lin was on hand to assist. These cells could theoretically form tumors when injected into humans. Now that he saw how enthusiastic Jun was about the nude mice, he was sure that this young man was not trying to commit murder.

After all the five nude mice were injected, Gu Jun put them into a breeding cage to see if they could form tumors.

"Senior brother, thank you very much." Gu Jun gave Senior Brother Zhang a thumbs up. Without the help of Senior Brother, it would not have been so smooth.

"The old Chinese doctor you are talking about..." Zhang Lin still looked like Conan, "Isn't he really a liar?"

…

For the next week, Gu Jun continued his previous life rhythm, except that he went to the nude mouse room every day to see the cage of nude mice. Their feeding was taken care of by the management of the nude mouse room, and each animal paid 3 yuan for room and board per day.

money.

He has only completed three ordinary tasks this week, because those who need to treat diseases and save people cannot do it. Although the hospital is nearby, he is not a doctor yet. The tasks he completed are all related to medical exercises, and the pills in his hand are already there.

5 boxes, and the proficiency of Tairan Hand has reached the first level (3000/5000).

On the seventh day, Gu Jun once again walked into the nude mouse room wearing sterile equipment and observed the nude mice one by one.

None of them are dead. A round tumor the size of a soybean has grown under the skin on the right side of No. 1’s buttocks. It is measured to be 0.5cm in diameter. No. 3 and No. 4 also have obvious protrusions, with diameters of 0.3cm and 0.3cm respectively.

0.5cm; No. 2 and No. 5 still showed no signs of tumor formation.

At this point, he was able to determine that out of the five nude mice, three had tumors.

Gu Jun set two nude mice with tumors as group a, one nude mouse with tumors as group b, two nude mice without tumors as group c and group d, and went to the animal center to buy 10 healthy nude mice.

, 5 are set up in group E, and 5 are set up in group f. Group a, group c and group e will all receive the same dose of "exotic medicine" (named by him) every day; while group b, group d and group f will all receive

There will be no medication, it will only be used for control purposes.

That is, nude mice with tumors, nude mice without tumors, and healthy nude mice each have a group that receives medication and a group that does not.

At this time, six cages have been divided on the experimental platform, and the naked mice are chirping and having fun.

Gu Jun was tinkering with 25 capsules of Yiwen Medicine. Since the system's instructions are that one box can extend life by 5 days, and there are 5 capsules in a box, it should be one capsule a day. This is the dose for adult humans, and the weight of these nude mice

The average is less than 20g. If they take one pill a day, they will probably be sacrificed immediately.

He poured out all the powder from a capsule and weighed the dose, then converted the dose to the nude mouse using his own body weight, prepared a solution, and then diluted it in proportion.

Soon, Gu Jun held the gavage needle filled with the medicine in one hand and a fixed healthy nude mouse in the other hand, and began to administer the gavage to the first mouse.

"Chirp chirp." The naked mouse struggled a little, but the poor thing was still given this injection of medicine by him, making its contribution to medicine.

At this moment, Gu Jun's heartbeat was a little fast, and he stared at the naked mouse in his hand without blinking.

He had never done a toxicity test on the exotic medicine until this moment. If it died suddenly, maybe the experiment would be meaningless. And now, he stared at it for five minutes, then 10 minutes...

Half an hour later, the naked mouse still showed no abnormality.

"It seems that the exotic medicine has no obvious acute toxicity." Gu Junsong breathed a sigh of relief, "We still need to further observe the chronic toxicity."

He then continued to administer medicine to the next nude mouse. After taking ten minutes to complete the administration of medicine to the other seven nude mice in the three drug groups, he observed for another hour. They all had no abnormal reactions and were still able to move. It barks, can drink water and eat feed.

"Every rat friend, please stay alive." Gu Jun muttered, pressing his aching head. His condition was obviously getting worse day by day, "Please."

…

On the second and third days, Gu Jun continued to work in the nude mouse room every day.

The tumors of the nude mice in group B that had not been given medication had increased from 0.3cm to 0.6cm, and their weight and mental state had not changed. However, in the two nude mice of group A that had been given medication, after two days, the size of the tumors was the same. There is no change and remains at 0.5cm.

Gu Jun knew what this might mean. The exotic medicine was really effective! These capsules could significantly inhibit the growth of tumors.

Moreover, everything was normal for nude mice administered other drugs, which also means that the exotic drug seems to have no adverse reactions and does little damage to body functions.

"Perhaps, there is really hope this time?" He thought to himself, although the uneasiness was still lingering in his heart, but a new hope was burning at the same time.

The fourth day, the fifth day...a week passed in the blink of an eye.

The tumor of the nude mouse in group b had grown to 1.5cm. The huge tumor was in sharp contrast with its thin body. It began to lose weight and became mentally ill. The two nude mice in group a continued to be in stable condition. All the nude mice treated with the drug lived well and were no different from the nude mice in the blank control group.

I have to say that the results obtained from this experiment are very satisfying.

The efficacy of heterogeneous drugs is even better than the top tumor small molecule targeted drugs currently on the market.

However, Gu Jun also knows that the adverse reactions of the exotic medicine cannot be completely ruled out. This only proves that it has no side effects in a short period of time. Moreover, this is an animal experiment after all, which is somewhat different from human clinical experiments.

However, the results of this experiment were worth his risk.

That afternoon, after Gu Jun wrote his suicide note, he went to the waiting area of ​​the emergency department of Dongda Affiliated Hospital and sat down on a rare waiting bench. The surrounding area was crowded with patients and their families, most of them crying children. With comforting parents, nurses hurriedly walked around, and caregivers pushed the rescue beds...

There was a commotion, and the radio in the emergency department kept calling: "No. 182, Chen Ziyang. No. 183, Wang Yuxuan. No. 184, Huang Zixuan..."

After exhaling, Gu Jun resolutely threw a Yiwen medicine capsule into his mouth, took a sip of mineral water and swallowed it.
Chapter completed!