"Professor Yang, we now want to do a batch of comparative experiments to improve the success rate." Su Fan and Wang Liangcai prepared for several days before going to find Yang Rui.

Although I work in a laboratory, the laboratory manager is not easy to disturb him casually. He has no dry goods in his stomach and is easily bullied.

Yang Rui is in charge of the laboratory and is not pretending to be a good person.

Even if it was the project of cloning sheep that he started, he would not do every experiment by himself. In fact, for such a large-scale project, his task is to supervise the completion of key points. As for those small parts, it is not worth investing in energy.

Therefore, Yang Rui did not pay any attention to the issues of Su Fan and Wang Liangcai. He nodded calmly and said, "It's great."

Originally, doing a comparison experiment is the only way. If Yang Rui still teaches this, this team would be too weak.

Su Fan had long been familiar with Su Fan's personality and smiled and said, "Professor Yang, we have some ideas on comparative experiments, so I would like to ask you to take a look."

"Oh? Which ones?" Yang Rui was still willing to do such specific questions.

"First of all, in terms of pretreatment solution. Gu Qiang used 3% sucrose, which seemed to be very effective, but he couldn't tell why. Considering that the sucrose solution may cause certain chemical damage to oocytes, we think it is necessary to conduct some investigation of the proportion of sucrose solution." Su Fan asked the first question in some tentative manner.

Yang Rui nodded and said, "The idea is right, what's the problem?"

He has automatically and consciously placed himself in the perspective of the problem solver.

When Su Fan heard that there was a show, he hurriedly said, "I wonder if I can ask the laboratory to organize a group, or add some funding staff to do this comparison experiment."

"Um..." Yang Rui was a little hesitant about continuing to increase resources to Su Fan and Wang Liangcai's group.

Their progress is not slow, but such a steady style is actually not very recognized by Yang Rui.

With the current domestic economic situation, even Yang Rui's laboratory cannot get international average funds. It can only be said that the lower price level has reduced the operating expenses of the laboratory. However, many experiments point out that foreign exchange is used, and the funds are always tight. If every problem is solved with resources, it will definitely be weak in the future.

"It's just a pretreatment solution, just start a research team, it's a bit of a big deal." Yang Rui pondered for a moment and expressed his objection.

When changing to a local university and doing some research for this, it seems to be a good topic. Many scholars probably don’t mind investing a year or two here.

But from Yang Rui's perspective, there are too many steps to cloning sheep, and the pretreatment solution is just a small point that cannot be smaller. What can be done if you study thoroughly?

Su Fan tried his best to put the expenses into the bottom line, saying: "In terms of pretreatment solutions, it should be almost the same to add 100,000 to 200,000 yuan to increase the funds."

If you save some, 200,000 yuan can still be studied relatively clearly in the pre-treated operating fluid, and 100,000 yuan of funds is just a joke.

Yang Rui even shook his head.

200,000 yuan is no less. Although the total amount of funds he received is eight digits, each project and research team spends money at the turnover price. It costs 200,000 yuan just for a pre-treatment operation fluid, which is not a good example.

Ultimately, when doing projects in the country, the right way is to save money if you can.

"I think your development direction can be adjusted." Yang Rui thought for a moment and pointed out: "Your problem is that you are worried that sucrose solution will cause chemical damage to oocytes, so you want to choose a solvent with as little damage as possible, right?"

"Yes. We hope to increase the success rate as much as possible. The chemical damage caused by sucrose solution is difficult to estimate. We are worried about the impact on the success rate in the later stage." Wang Liangcai quickly explained to Yang Rui.

Yang Rui nodded and said, "It is not difficult to understand that you have any worries in this regard, but the degree of chemical damage is difficult to verify."

"Yes..." Wang Liangcai was a little helpless.

"I think this issue should be considered from two aspects." Yang Rui spoke slowly.

When it comes to pointing out the experimental direction, Yang Rui has always been generous and has no much scruples.

Seeing the direction from the chaos and driving in the fog is what scholars do.

If you do well, you will only get praise, not doubt.

Wang Liangcai also suddenly became energetic and stared at Yang Rui with his breath.

Yang Rui thought about it and said, "First, let's talk about pre-treatment of operating fluids. You can not only consider it from the perspective of reducing chemical damage, but also from the perspective of protecting oocytes."

Wang Liangcai and Su Fan looked at each other without saying a word. This was one of the obvious strategies, but the strategies may not be realized.

Yang Rui tapped the table with his finger twice, and decided to say it more clearly, saying: "If I were doing this experiment, I would definitely not use a simple sucrose solution. I would consider giving oocytes a more adaptable environment, for example, fetal bovine serum is a good choice."

"Feal cattle serum..." Wang Liangcai didn't expect that Yang Rui could really speak, and it sounded quite reasonable.

In genetics laboratories, serum is also a common reagent, and fetal bovine serum will not be unfamiliar. However, if it were not for Yang Rui's suggestion, Wang Liangcai and Su Fan, who were full of sucrose solution, would not have thought of it.

In fact, in the entire laboratory, including Gu Qiang's group, no attempt was made to add fetal bovine serum to the operating fluid.

"Let's try it." Wang Liangcai was a little excited.

If you judge only from experience, adding fetal bovine serum is also a very reliable choice.

"Director, what about the second point?" Su Fan also felt it was reliable. He had been doing cell cloning for a long time and had used fetal cattle serum. Now, if you think about it, all indicators are very consistent.

Yang Rui smiled and said, "The second point is, I think your ideas need to be changed."

"yes."

"Our goal is to clone sheep, huh, clone sheep that is sexually bred... In short, this project is not a commercial project for the time being, so we only need to make a clone sheep in the end, with unlimited success rate and not cost-effective."

Yang Rui said it seemed very easy. Just cloning a sheep or something, it was helpless to hear Wang Liangcai and Su Fan.

But what Yang Rui said makes sense. If the funds are sufficient and the staff are sufficient, pushing them from bottom to top will naturally be the easiest model.

However, it is basically unlikely that sufficient funding and sufficient staffing are sufficient.

There are few scientific research projects that allow scholars to consume resources at will.

From Yang Rui's perspective, cloning sheep is a bottomless pit project.

Even in another thirty or forty years, people will have only a little understanding of the various key points in cloning.

Historically, the Scots conducted more than 600 sets of experiments to make a cloned sheep, Dolly. If their previous research was very sufficient, even through insurance measures, two or three cloned sheep might eventually be born.

It can only be said that the appearance of the cloned sheep Dolly also has a certain component of luck.

Before it, there were so many cloned sheep that had miscarried, and countless time and energy were wasted.

If Yang Rui does not want to spend the next ten years on the same project, it is imperative to adopt a more economical approach.

Studying every key point thoroughly is neither a correct choice nor impossible to achieve, and it is even more unnecessary.

As long as Yang Rui's laboratory can complete the project of cloning sheep, there will naturally be people who will find out the missing items without clear research on the previous loopholes.

So many local universities, young people who have just debuted, and poor laboratories in the Third World, have done this kind of work all their lives, and they are happy and have to give them some opportunities.

Su Fan and Wang Liangcai also understood.

Yang Rui obviously disagrees with their step-by-step model. Wang Liangcai couldn't help but feel a little anxious and said hurriedly: "Professor Yang, Director Yang, cloning sheep is a series of projects. If the success rate in the early stage cannot be guaranteed, he will not even be qualified to enter the later stage."

"Didn't Gu Qiang bet on the core count?"

"We can't learn Gu Qiang's method. He himself knows the reason but not the reason." Wang Liangcai sighed.

Yang Rui said calmly: "Then you know why, but you don't know why."

"ah?"

"We are in the same laboratory, in the same project team, and are divided into different research teams, just to try different paths. Since Gu Qiang has mastered one, then use it. Why spend the experience on opening up a parallel path? My suggestion, until the part of the reimbursement, you will only carry out the work of checking and filling the gaps, starting with reimbursement, and continuing to make efforts."

Wang Liangcai was stunned when he heard this, but felt enlightened.

"You are right, there is no need to worry about why you use 5% concentration, 5% concentration is easy to use." Wang Liangcai couldn't help but knock on his head.

Has anyone made an experiment very thorough?

Of course there are, but those who do this step are usually studied from the back to the front, that is, the results of future generations studying the predecessors.

Scholars who are truly doing pioneering research and standing at the forefront of science are like foreigners exploring in the primitive rainforest. Who has the energy and time to explore the varieties and characteristics of each tree? Who has the ability to figure out the life under every inch of soil, or observe the movements in every canopy?

Doing scientific research is like driving in a foggy day. You can’t stop, it’s waiting to be hit by the car behind. Don’t expect how far you can visibility you can be. You can see the road under your feet. It’s good if you don’t fall down.

Although Gu Qiang's experimental report lacks detailed analysis, one thing is fine. He at least proves that the road is clear.

"The success rate still needs to be, but there is no need to pursue the limit. It's better to solve the problems or not." Yang Rui was equally overturned the original idea of ​​Wang Liangcai and Su Fan, but the two were not unhappy.

You have to listen to valuable suggestions, which is the minimum attitude of scientific research.

The trick of using strength with cells is useless, and it is even more trouble for yourself if you can't get through with the funds.

Wang Liangcai thought about it on his own, and after a moment, he said, "Since that's the case, let's repeat Gu Qiang's experiment first, and then pick out the obviously low success rate part of it for in-depth research."

"In this case, Gu Qiang will lead the way for a while." Su Fan was slightly unwilling.

Wang Liangcai glanced at Su Fan and said, "It's not fast to be fast first. Cloning sheep is a big project. Just like long-distance running, we will implement the following strategy."

Compared to Su Fan, Wang Liangcai's personality is obviously more calm, and his method is the best way to take advantage of the two of them.

Moreover, compared to the stupid way to build foundations, the revised plan also takes into account both success rate and speed.

The most important thing is that Gu Qiang's advantage has also indirectly become the advantage of the two.

Yang Rui also nodded secretly, as the laboratory's efficiency can be greatly increased.

No matter how lucky Gu Qiang is, he cannot really get straight to the answer. Yang Rui always needs to correct his direction from time to time. The most important thing is that the lack of theoretical reserves makes Gu Qiang's scientific research expansion ability very weak.

Such as backtracking experiments, checking for missing and even increasing success rate and improving experimental stability, are not what Gu Qiang is good at.

But these abilities are indispensable in such a long project line as cloning sheep.

Wang Liangcai is just the opposite. He is not a scholar who is good at creating surprises, but a stable long-distance runner. If he lets go of a little bit of insignificant face, what Wang Liangcai gets is the opportunity to strengthen the fast running.

"Professor Yang, we also want to apply for more aircraft, especially at night." Wang Liang paused and said, "Can we give us all aircraft after the early morning?"

"If you apply for the night time, you must come every night." Yang Rui asked: "Can your body stand it?"

"I can take a two-hour nap at noon and another four hours after supper. In addition, I can sleep about two hours the next morning, so there is no problem." Wang Liangcai said relaxed, but the three of them all knew how cruel perseverance it was to spread the entire sleeping time.

"Don't you need some...private time?" Yang Rui has seen those who work hard and often asks the experimenters to work hard. However, it is rare to take the initiative to ask for extra long working hours.

Wang Liangcai grinned and said, "I don't have my family here, what do I need to do in my private time."

Yang Rui was speechless and shrugged, saying, "It's good. Since your family is not in the capital, you can eat a small stove in the cafeteria. I asked the nutritionist to prepare a meal for you to ensure that it tastes good and has sufficient nutrition. By the way, I asked a masseur a while ago, a national level. When someone arrives, I will allocate an hour to you every day."

Su Fan's envious saliva was about to flow down, but Wang Liangcai himself was confused.
Chapter completed!